Cell Culture Toolkit

CELL CULTURE TOOLKIT v0.9.0

Click preset to add · Custom cell lines saved to this browser · Bundle includes cell-line data + media recipe

Sync Time

Schedule Day Duration Auto

Passage Schedule Passage Time

Allow intermediate passages (multi-hop)

Settings

Target Confluence at Sync (%) Min Seeding Safety Margin (×min density)

Biz Hours Start

Biz Hours End

Allow Weekend Passages

Allow Out-of-hours Passages

Literature References

Model Assumptions

Growth model: logistic or Gompertz with lag offset, auto-selected by R².
Logistic: C(t) = K · C₀ / (C₀ + (K − C₀) · e^{−r(t − τ)})
Gompertz: C(t) = K · exp(−ln(K/C₀) · e^{−r(t − τ)})

Confluence ↔ density: 100% ≈ 1.5 × 10⁵ cells/cm² (approximate, varies by cell size).

Passage number: DT multiplier per passage from senescence trend. Values are heuristic.

Add cell lines above — results update automatically_

Cell Line

Name

Doubling Time (h)

Advanced (Lag / Plateau / Density)

Lag (h)

Plateau (%)

Cells/cm² at 100%

Plating Vessel

Format

Count

Media / Well (mL)

Surface area

Cell Stock Source

From Flask Known Cells/mL

Source Flask

Source Confluence (%)

Resuspended Stock Volume (mL)

Trypsinise flask → resuspend → transfer fraction to plating media.

Timeline

Day 0 (Plating Day)

Plate at

Checkpoints

Mark one as primary — we back-calculate seeding to hit that confluence.

Pick cell line, vessel, and at least one checkpoint, then click Plan Plating_

Cell Line

Name (optional — matches preset)

Optional Metadata

Passage #

Day 0 Date

Passage Setup

Source Confluence (%)

Target Seeding (%)

Passage Method

Trypsin → resuspend Direct dilution

Stock Vol (mL) trypsin+media

New Flask Vol (mL)

▸ PIPETTE THIS

Grow to (%) (passage threshold)

Enter target seeding % above ↑

Verify actual transfer

Transfer Vol (mL)

Media Added (mL)

New total: → Seeding: -

Observations

Hours post-passage	Confluence (%)

Advanced (Lag / Plateau)

Lag Phase (h)

Plateau (%)

Auto-filled from literature if cell line name matches a preset.

Enter passage details and add at least one observation — results update automatically_

Import a file and select a cell line to view its full measurement history.

Bundle (cell + media data):

Media Prep

Preset Libraries

Mammalian Bacterial

Tick to enable preset libraries in dropdowns. Bacterial loaded via Bundle Import.

Total Volume (mL)

Base Medium(s)

Multiple bases share non-additive remainder by ratio.

Additives / Components

Protocol

Notes

Configure media parameters and press Calculate_

⚙ DEV PANEL

idle

← ASTRELT.EXE